The methodology of soil fertility assessment in the software product Maplnfo 60 is developed. This assessment establishes the true suitability of land for cultivation of various crops and is associated with the assessment of not only productive properties of land, but also economic, which makes the problem urgent. As the initial data we used the plan of the agricultural enterprise and the results of soil sampling. By means of the geoinformation system (GIS) Maplnfo the GIS-project was created, which included the raster image, the results of the digital description of the objects and the semantic database. Spatial data analysis using Maplnfo tools was performed. Maps of farm roads, non-agricultural and agricultural land were created, as well as a composite general map of all lands of the enterprise. The average weighted scores of soil goodness by agricultural lands, by diagnostic features of soils and by yield of main crops were calculated, as well as the correlation dependence between the yield of soils and the content of nutrients in them was established. The developed method allows to carry out an assessment of soils taking into account more factors, it was possible so far, and can be used in scientific, practical and educational purposes.